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. 2025 Jan 6;15:954. doi: 10.1038/s41598-024-82109-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — Invasion and apoptosis assay. (A) Transwell assay showing that knockdown of SLCO4A1-AS1 reduced the invasive ability of LN229, A172, and U118 cells (n = 3). Scale bar = 100 μm. (B) Quantitative analysis of the transwell assay results to assess the invasion ability of glioma cells following SLCO4A1-AS1 knockdown. (C) 3D tumor sphere invasion assay to assess the invasive capacity of GBM#P3 after knockdown of SLCO4A1-AS1 (n = 3). Scale bar = 100 μm. (D) Quantitative analysis of the 3D tumor sphere invasion assay results to assess the invasive ability of GBM#P3 following SLCO4A1-AS1 knockdown. (E) Annexin V staining of LN229, A172, U118, and GBM#P3 cells following SLCO4A1-AS1 knockdown (n = 3). (F) Quantitative analysis of Annexin V staining in LN229, A172, U118, and GBM#P3 cells following SLCO4A1-AS1 knockdown. Data are presented as the mean ± SD. NS, not statistically significant; * P < 0.05; ** P < 0.01; *** P < 0.001. 1-way ANOVA was used for data analysis.