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. 2025 Jan 7;8:0574. doi: 10.34133/research.0574

Fig. 3.

Fig. 3.

Knocking down tRF-AspGTC alleviates hydrogen peroxide-induced VSMC phenotypic switching, oxidative stress, and inflammation. (A) Knockdown efficiency of tRF-AspGTC inhibitor transfection detected by RT-qPCR; n = 3 per group. (B) WB analysis of the expression levels of contractile VSMC marker proteins MHC, CNN1, and α-SMA in VSMCs treated with 400 μM hydrogen peroxide for 24 h following tRF-AspGTC knockdown. (C) Quantitative analysis of (B); n = 3 per group. (D) WB analysis of MMP2 and MMP9 protein expression levels. (E) Quantitative analysis of (D); n = 3 per group. (F) RT-qPCR analysis of MMP2 and MMP9 mRNA levels; n = 3 per group. (G) RT-qPCR analysis of the mRNA levels of inflammatory cytokines IL-1β, TNF-α, and IL-6; n = 3 per group. (H) Detection of ROS generation in VSMCs. **P < 0.01; ***P < 0.001; ****P < 0.0001.