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. 2025 Jan 7;8:0574. doi: 10.34133/research.0574

Fig. 6.

Fig. 6.

tRF-AspGTC accelerates VSMC phenotypic switching, oxidative stress, and inflammation by targeting galectin-3. (A) RT-qPCR analysis of LGALS3 mRNA levels in human STA and IA tissues; n = 8 per group. (B) RT-qPCR analysis of LGALS3 mRNA levels in the brain arteries of normal and IA mice; n = 6 per group. (C) WB analysis of galectin-3 expression levels in human STA and IA tissues. (D) Quantitative analysis of (C); n = 3 per group. (E) Immunohistochemical (IHC) detection of galectin-3 expression in human STA and IA tissues. (F) IHC detection of galectin-3 expression in the brain arteries of normal and IA mice. (G and H) Quantitative analysis of (E) and (F); n = 5 per group. (I) WB analysis of the expression levels of contractile VSMC marker proteins MHC, CNN1, and α-SMA in VSMCs treated with hydrogen peroxide following LGALS3 knockdown. (J) Quantitative analysis of (I); n = 3 per group. (K) WB analysis of MMP2 and MMP9 expression levels. (L) Quantitative analysis of (K); n = 3 per group. (M) RT-qPCR analysis of MMP9 and IL-1β mRNA levels in VSMCs treated with hydrogen peroxide following LGALS3 knockdown; n = 3 per group. (N) RT-qPCR analysis of MMP9 and IL-1β mRNA levels in VSMCs transfected with mimic-NC or tRF-AspGTC mimic and si-NC or si-LGALS3, followed by hydrogen peroxide treatment; n = 3 per group. (O) WB analysis of CNN1, α-SMA, and MMP9 expression levels. (P) Quantitative analysis of (O); n = 3 per group. (Q) Detection of ROS generation. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.001. IOD, integrated optical density; si-LGALS3, small interfering RNA targeting lectin galactoside-binding soluble 3.