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Single cells’ genetic expression and environment (a - untreated) cause inherent dissimilarities within conditions, challenging perturbation detection and measurement. The figure (b - high concentration Nocodazole-treated cells) shows four morphological responses to the same treatment, one resembling untreated cells (b - far right). Most lower-concentration treatments produce phenotypes visually similar to untreated cells (data not shown). Images in this dataset are centered on a cell.
