Table 1. Primer sequence for real-time quantitative PCR.
| Gene | Forward (F)/Reverse (R) | Sequence | GenBank accession | |
|---|---|---|---|---|
| Bcl6b | B-cells CLL/lymphoma 6 member B protein | F (5′-3′) | TACTTCAAGGCTTCGCCTCTCT | NM_007528 |
| R (5′-3′) | CTACGTGTTCCATCTGCAAATAGG | |||
| Bmi1 | B cells-specific Moloney murine leukemia virus integration site 1 | F (5′-3′) | TGTCCAGGTTCACAAAACCA | NM_007552 |
| R (5′-3′) | TTGAAAAGCCCTGGGACTAA | |||
| Ddx4 (VASA) | DEAD-box helicase 4 | F (5′-3′) R (5′-3′) |
GCTTCATCAGATATTGGCGAGT GCTTGGAAAACCCTCTGCTT |
NM_010029 |
| Id4 | DNA-binding protein inhibitor ID-4 | F (5′-3′) | CAGTGCGATATGAACGACTGC | NM_031166 |
| R (5′-3′) | GACTTTCTTGTTGGGCGGGAT | |||
| Gfrα 1 | GDNF family receptor alpha 1 | F (5′-3′) | CACTCCTGGATTTGCTGATGT | NM_010279 |
| R (5′-3′) | AGTGTGCGGTACTTGGTGC | |||
| Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | F (5′-3′) | CTGACGTGCCGCCTGGAGAA | NM_001289726 |
| R (5′-3′) | CCCCGGCATCGAAGGTGGAA | |||
| Kit | KIT proto-oncogene receptor tyrosine kinase | F (5′-3′) | GCCACGTCTCAGCCATCTG | NM_001122733 |
| R (5′-3′) | GTCGCCAGCTTCAACTATTAACT | |||
| Lhx1 | LIM homeobox 1 | F (5′-3′) | CCCAGCTTTCCCGAATCCT | NM_008498 |
| R (5′-3′) | GCGGGACGTAAATAAATAAAATGG | |||
| Pcna | Proliferating cell nuclear antigen | F (5′-3′) | TGTTTGAGGCACGCCTGATCC | NM_011045 |
| R (5′-3′) | GGAGACGTGAGACGAGTCCAT | |||
| Zbtb16 (Plzf) | Zinc finger and BTB domain containing 16 | F (5′-3′) | CTGGGACTT TGTGCGATGTG | NM_001033324 |
| R (5′-3′) | CGGTGGAAGAGGATCTCAAACA |