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. 2025 Jan 6;15:893. doi: 10.1038/s41598-025-85528-8

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — RT-qPCR Analysis of TLR3, MDA5, and RIG-I Gene Expression in THP-1 Cells Transfected with Specific miRNAs. This figure shows the results of our RT-qPCR analysis measuring the expression levels of TLR3, MDA5, and RIG-I genes in THP-1 cells following transfection with different miRNA mimics: let-7e-5p, miR-146a-5p, miR-27b-3p, miR-151a-5p, let-7d-3p, and miR-130a-3p. Quantification data were normalized to the expression of the housekeeping gene GAPDH. The Y-axis represents the fold increase in specific mRNA levels over the control samples (NC: non-targeting mimic control, mimic; each mimic of let-7e-5p, 146a-5p, 27b-3p, 151a-5p, let-7d-3p, 130a-3p miRNA). Data are presented as the mean ± standard error of the mean (SEM) from three independent experiments, with each group consisting of four replicates. Statistical significance is indicated by asterisks: *p < 0.05, **p < 0.005, ***p < 0.0005, highlighting differences in gene expression induced by each miRNA mimic compared to the control.