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. 2025 Jan 6;16(1):4. doi: 10.1038/s41419-024-07320-2

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© The Author(s) 2025

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Fig. 2 — A Sequence alignment of the p53 family linker region. The core region of the linker (based on sequence homology to the p53 linker region) is shaded in grey. B Pull-down assay of p53 family member isoforms with GST-tagged ASPP family CTDs and GST alone as bait. N terminally Myc-tagged proteins were in-vitro translated using RRL. Input and pull-down (PD) samples were analysed by WB using an α-Myc antibody. The bait protein in the PD samples was detected by ponceau staining of the WB membrane.