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. 2025 Jan 6;16:335. doi: 10.1038/s41467-024-55330-7

Fig. 2. Prognostic relevance of JUNB-repressed inflammatory signaling.

Fig. 2

Coverage of previously published21 JUNB ChIP-seq data in CAPAN1, as well as publicly available H3K27ac25 data, for loci of JUNB (a), GATA6 (b), HNF1B (c), and FOXA1 (d). ChIP-qPCR validation regions are indicated. ChIP-qPCR for regions indicated in (a–d), showing signal relative to input for JUNB (e) and H3K27ac (f) pulldown with mean ± s.d. and average IgG isotype control. n = 3 biological replicates. g–i Integration of RNA-seq data performed after JUNB silencing (siJUNB; n = 3 biological replicates) or control siRNA (siCtrl; n = 2 biological replicates) in CAPAN1, with ChIP-seq for JUNB. g Violin plot of log2 fold change (FC) in siJUNB RNA-seq data for all (n = 36.740) or JUNB-bound (n = 698) genes. Median and quartiles are indicated. Two-tailed Student’s t-test with Welch’s correction. h As in (g), showing the number of genes that display a significant upregulation (sigUP) or downregulation (sigDN), or no significant change (ns). i Gene ontology analysis of significantly upregulated, JUNB-bound genes following JUNB silencing with –log10(q-value) indicated. Hallmark (H) and curated (C2) signature collections of the Molecular Signature Database (MSigDB) are shown. j Spearman correlation of genes as in (i) with JUNB in 46 PDAC cell lines of the Cancer Cell Line Encyclopedia (CCLE). Negatively associated genes (red) form the JUNB repression signature. k Overall survival, numbers at risk, and hazard ratio in TCGA (n = 150), Puleo (n = 288), QCMG (n = 96), and Zhou (n = 85) patients stratified by JUNB repression signature (j) score. Top: Kaplan-Meier survival analysis for the lower/upper quartiles (n = 155 patients each) and mid-group (n = 309 patients) for JUNB repression signature scores. Median survival (ms) is indicated. Log-rank test. Bottom: Cox proportional hazard. Hazard ratio (to lower quartile) with 95% CI. P values are shown right. l As in (k), for progression-free survival in the TCGA cohort. Source data are provided as a Source Data file.