Fig. 3.

6-OHG inhibited hypoxia-stimulated oxidative stress in PC12 cells. (A) The ROS level measured by DCFH-DA on a fluorescence microscope. MDA content (B), SOD activity (C), CAT activity (D), and GSH level (E) in the cell lysis supernatant were determined by corresponding kits. (F) The protein expression levels of HIF-1a and VEGF were detected by Western blotting. (B) Quantitative analysis of the expression of HIF-1a (G) and VEGF (H) proteins. Data are expressed as mean ± SEM (n = 3 or 4). Statistical analysis was performed using one‑way ANOVA corrected with Tukey’s test. ^#p < 0.05 or ^##p < 0.01 versus Nor group; ^*p < 0.05 or ^**p < 0.01 versus Hy group.