Fig. 5.

6-OHG inhibited hypoxia-stimulated apoptosis in PC12 cells. (A) The apoptosis of PC12 cells was detected by TUNEL staining (Magnification, × 20). The activities of Caspase-3 (B) and Caspase-9 (C) was measured by corresponding kits. (D) The protein expression levels of Bax, Bcl-2 and cleaved caspase-3 were detected by Western blotting. Quantitative analysis of the ratio of Bax/Bcl-2 (E) and the expression of cleaved caspase-3 (F) protein level. Data are expressed as mean ± SEM (n = 3 or 4). Statistical analysis was performed using one‑way ANOVA corrected with Tukey’s test. ^#p < 0.05 or ^##p < 0.01 versus Nor group; ^*p < 0.05 or ^**p < 0.01 versus Hy group.