Table 2. Sequences of exact complement and overhanging oligonucleotides, melting temperatures (Tm) with bisPNA-AEEA3 and differences in Tm between exact complements and overhanging oligonucleotides.
| Name | Sequence (5′→3′) | Tm (°C) | ΔTm (°C) | |
|---|---|---|---|---|
| Exact complements | DNA-1exact | tctaggtgaaga | 49.5 | - |
| DNA-2exact | caaatatgtatc | 45.6 | - | |
| Projecting outward | DNA-1outward | tctaggtgaaga(n)38 | 49.3 | –0.2 |
| DNA-2outward | (n)28caaatatgtatc | 48.1 | 2.5 | |
| Partial inward/partial outward | DNA-1mid DNA-2mid | (n)19tctaggtgaaga(n)19 (n)14caaatatgtatc(n)14 | 54 52.8 | 4.5 6.2 |
| Projecting inward | DNA-1inward | (n)38tctaggtgaaga | 54.4 | 4.9 |
| DNA-2inward | caaatatgtatc(n)28 | 51.9 | 5.4 |
n, a randomized nucleotide.