Fig. 5.

Histone modification-related protein expression in SNUC5 and SNUC5/RR cells. (A) Expression of histone methylation-related proteins, MLL1, EZH2, H3K4Me3, and H3K27Me3, and histone acetylation-related proteins, HAT1, HDAC1, and H3K9Ac, determined using western blotting. (B) Anti-MLL1 or -HAT1 antibodies and primers targeting the NRF2 promoter region used for the ChIP analysis. *p<0.05 vs. untreated SNUC5 cells (n=3). (C) NRF2 and HO-1 protein expression levels in SNUC5/RR cells transfected with siMLL1 or siHAT1 RNA for 24 h determined using western blotting.