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Effect of hydrogen peroxide on DNA polymerase activities of Exo–p140, pol β and pol α. All three enzymes were treated with H2O2 for 60 min and their DNA polymerase activities on poly(dA)·oligo(dT) were measured as described in Materials and Methods. Reaction mixtures contained ∼48 ng (0.4 pmol) Exo–p140 (open circles), 17 ng (0.4 pmol) pol β (open diamonds), ∼66 ng (0.4 pmol) pol α (filled squares), respectively.
