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Pentamidine alters the folding of Ca.LSU precursor RNA. The same amount of radiolabeled Ca.LSU precursor in Tris buffer was incubated at 0 or 37°C for 20 min in the presence of the indicated concentration of pentamidine and was then analyzed by native PAGE (Materials and Methods). The two different exposures were to allow clear visualization of the S and F bands (Exposure 1) and smear RNA species (Exposure 2).
