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MgCl2 antagonizes the alteration of folding of Ca.LSU precursor RNA induced by pentamidine. Equal amounts of radiolabeled precursor RNA were preincubated in Tris–HCl in the presence of 0, 25 or 100 µM pentamidine and in the absence or presence of 1.25 mM MgCl2, 1.25 mM CaCl2 or 0.4 mM spermidine at 37°C for 20 min, then analyzed by native PAGE as described in Materials and Methods.
