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. 2025 Jan 1;16(3):750–764. doi: 10.7150/jca.101050

Figure 6.

Figure 6

Knockdown of HIF-1α expression inhibits subcutaneous tumor formation. (A) Imaging of subcutaneous tumors in nude mice; (B) Changes in body weight of nude mice after subcutaneous tumor formation; (C) Changes in subcutaneous tumor volume; (D) Detection of ALT content in serum of nude mice after subcutaneous tumor formation; (E) Detection of AST content in serum of nude mice after subcutaneous tumor formation; (F) Detection of BUN content in serum of nude mice after subcutaneous tumor formation; (G) Immunoblotting showing HIF-1α and CD47 protein expression levels in subcutaneous tumor tissue; (H) Quantification of HIF-1α protein expression level; (I) Quantification of CD47 protein expression level. Data are presented as mean ± standard deviation, and differences between the two groups were analyzed using an unpaired t-test. **P <0.01, ***P <0.001, ****P <0.0001.