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. 2024 Dec 24;11:1500969. doi: 10.3389/fvets.2024.1500969

Figure 3.

Figure 3

miR-145 inhibited chondrocyte differentiation of RMCs. (A) The relative expression of miR-145 in RMCs with different treatments was evaluated by qRT-PCR. (B) Alisin blue staining of RMCs with different treatments. (C) Quantitative analysis of Alcian blue staining. (D) The expression of chondrogenic genes of RMCs with different treatments was analyzed using qRT-PCR. (E) IF of COL II of the RMCs with different treatments. (F) Quantitative analysis of IF of COL II. (G) IF of COMP of the RMCs with different treatments. (H) Quantitative analysis of IF of COMP. (I) IF of COL X of the RMCs with different treatments. (J) Quantitative analysis of IF of COL X. (K) The expression of chondrogenic proteins of RMCs with different treatments was analyzed using WB; (L) Quantitative analysis of WB.