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. 2024 Dec 24;11:1500969. doi: 10.3389/fvets.2024.1500969

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Copyright © 2024 Jia, Han, Li, Zhang, Ma, Wang, Wang, Yan, Li, Shen, Chen, Li, Zhang, Hu and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-145 targeted and suppressed SOX9 expression. (A) The target genes of miR-145 were predicted by online websites. (B) Venn diagram of differentially expressed target genes of miR-145 in the pairwise comparisons. (C) Clustering heatmap of 128 differentially co-expressed target genes of miR-145. (D) qRT-PCR analysis of SOX9 expression during RMC chondrogenic differentiation. (E) The expression trend of miR-145 and SOX9 was negatively correlated. (F) Normalized luciferase activity after co-transfection of miR-NC mimics or miR-145 mimics together with SOX9 3’UTR-WT or SOX9 3’UTR-MUT. (G) The expression of SOX9 of RMCs with different treatments was analyzed using IF. (H) Quantitative analysis of IF. (I) The expression of SOX9 of RMCs with different treatments was analyzed using qRT-PCR. (J) The expression of SOX9 of RMCs with different treatments was analyzed using WB. (K) Quantitative analysis of WB.