Table 2. Relative G-strand overhang signals from DT40 knockout cell lines Ku70^–/–, Rad52^–/–, XRCC2^–/–, XRCC3^–/– and DNA-PKcs^–/–

Cell line	G-overhang signal intensity (%)
Wild-type	100
Ku70–/– (TelL)	109 ± 12
Ku70–/– (TelS)	93 ± 12
Rad52–/–	96 ± 24
XRCC2–/–	106 ± 21
XRCC3–/–	89 ± 15
DNA-PK–/–	97 ± 22

The G-overhang signal was measured for each lane and normalized to the amount of genomic DNA loaded in that lane. The amount of overhang signal from each knockout cell line was compared with the signal from wild-type DT40 DNA run in duplicate on the same gel. Ku70^–/– (TelS), Ku70-deficient clones with short (∼3 kb) telomeres; Ku70^–/– (TelL), Ku70-deficient clones with longer (5–6 kb) telomeres. The standard deviations refer to the variation in relative G-overhang signal obtained from at least two DNA preparations run in duplicate on two different gels.