FIGURE 1.

Overview of staining approach. Literature review of neuromuscular junction structure helped guide prospective antibody targets for staining which further guided identification of suitable antibodies from manufacturers. Full Yucatan minipig larynges were harvested and transected at midline (denoted by line) to allow for full visualization of TA muscles. Harvested TA muscle samples were processed via formalin fixed paraffin‐embedded (FFPE) method into 5‐μm thick sections. Following rehydration utilizing an ethanol‐deionized water gradient, samples were stained with selected antibodies. Stained slides were then efficiently visualized on an Echo Revolve Microscope at 40× on fluorescent settings. Figure created in part with Biorender.com.