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. 2002 Jul 1;30(13):2736–2741. doi: 10.1093/nar/gkf400

Figure 2.

Figure 2

EMSAs of Klf4 and Klf5 on an additional cis-element, GC-2, that is present in the Klf4 promoter. EMSAs were performed with 10 µg nuclear extracts (N.E.) prepared from cells transfected with PMT3, PMT3–Klf4 or PMT3–Klf5 in the absence of any competitors (0), or in the presence of 25× molar excess of a cognate competitor (WT) or an unrelated competitor (AP1). Probes used include TDA (A), BTE (B) and GC-2 (C). * represents the shifted Klf4–probe complex and + represents the shifted Klf5–probe complex.