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. 2025 Jan 1;21(2):614–631. doi: 10.7150/ijbs.103032

Figure 7.

Figure 7

Increased expression of ACLY can counteract the effects of sh-LPCAT1 on ccRCC. A, B. Transwell assay was used to verify changes in the proliferation and migration abilities of transfected cells, and ImageJ was used to quantify the migrating cells (scale bar:100μm). C. Wound healing assay was measured to verify the cells' migration ability, and the wound healing status was observed after 24 hours. D. The growth curve drawn using the CCK-8 assay shows that the slowed cell proliferation caused by LPCAT1 knockdown can be alleviated by increased expression of ACLY. E. The triglyceride content in transfected cells was measured using a micro-TG assay kit. F. BODIPY 493/593 was used to detect changes in neutral lipids in transfected cells. Green indicates lipid droplets. Images were taken using an Olympus microscope(scale=20μm). G. Flow cytometry was used to quantitatively analyze changes in lipid droplet content in different transfected cell lines, using cells without BODIPY 493/503 as a blank control.