Fig. 3.

Characterization of light transmission aggregometry (LTA) curves for the association and dissociation of MDA‐MD‐231 cancer cells with low (50 000 platelets·μL⁻¹), normal (200 000 platelets·μL⁻¹), and high (450 000 platelets·μL⁻¹) concentrations of platelets. The LTA curves for the MDA‐MB‐231 tumor cell association, and dissociation in presence of platelets after treatment with different concentrations of tissue plasminogen activator (tPA) and tenecteplase (TNK), were analyzed to produce an average curve (A–C), determine the dissociation lag time (D–F), and measure the time to 25% disassociation (G–I). The tPA and TNK conditions were: control (all platelet concentrations: n = 4), 1 μg·mL⁻¹ tPA (50 000 platelets·μL⁻¹: n = 3, 200 000 platelets·μL⁻¹: n = 3, 450 000 platelets·μL⁻¹: n = 4), 0.5 μg·mL⁻¹ tPA (50 000 platelets·μL⁻¹: n = 3, 200 000 platelets·μL⁻¹: n = 3, 450 000 platelets·μL⁻¹: n = 4), 0.25 μg·mL⁻¹ tPA (all platelet concentrations: n = 3), 1 μg·mL⁻¹ TNK (50 000 platelets·μL⁻¹: n = 3, 200 000 platelets·μL⁻¹: n = 3, 450 000 platelets·μL⁻¹: n = 4), 0.5 μg·mL⁻¹ TNK (50 000 platelets·μL⁻¹: n = 3, 200 000 platelets·μL⁻¹: n = 4, 450 000 platelets·μL⁻¹: n = 4), and 0.25 μg·mL⁻¹ TNK (50 000 platelets·μL⁻¹: n = 3, 200 000 platelets·μL⁻¹: n = 4, 450 000 platelets·μL⁻¹: n = 4). For each condition, the treatment is added at 10 min as indicated with a dotted line on the average curve (A–C). The lag time (D–F) and time to 25% dissociation (G–I) are measured from the addition of the treatment. To determine the statistical significance of differences between conditions, a one‐way ANOVA analysis with Tukey's multiple comparison test was performed (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). For all conditions, the mean and standard error of the mean (SEM) are displayed.