Fig. 3.

BMAL1 knockdown aggravated Alzheimer-like pathology. A, B Western blots and quantitation analysis of the phosphorylation levels of tau at Ser396, Thr231, and Ser199 (n = 4 per group). C, D Western blots and quantitation analysis of the expression levels of APP and BACE1 (n = 4 per group). E, F Immunohistochemical for Aβ plaques in the hippocampus of mouse and quantitation analysis in four groups (Scale bars = 400 µm in low-magnification and scale bars = 100 µm in high-magnification image) (n = 3 per group). G ELISA of Aβ1-40 and Aβ1-42 in the hippocampus (n = 4 per group). Data were plotted as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. The data were analyzed by two-way ANOVA followed by post hoc Tukey’s HSD test