TABLE 3.
Phenanthrene biotransformation products in the strain MA-2 cultures identified by liquid chromatography-high-resolution mass spectrometry analyses
| Product | Proposed identity | Chemical formula | [M – H]− | Retention time (min) | Retention time of standards (min) | Observed m/z | Theoretical m/z | Mass error (ppm) |
|---|---|---|---|---|---|---|---|---|
| I | o-Hydroxynaphthyl-α-oxobutenoates | C14H9O4− | 241 | 6.0 | –a | 241.0506 | 241.0506 | −0.1531 |
| II | 1-Hydroxy-2-naphthoic acid | C11H7O3− | 187 | 5.9 | 5.8 | 187.0396 | 187.0401 | −2.4626 |
| III | 1,2-Dihydroxynaphthalene | C10H7O2− | 159 | 6.4 | 6.5 | 159.0451 | 159.0452 | −0.4811 |
| IV | 2-Hydroxybenzalpyruvic acid | C10H7O4− | 191 | 3.7 | – | 191.0349 | 191.0350 | −0.6445 |
| V | Salicylaldehyde | C7H5O2− | 121 | 4.5 | – | 121.0293 | 121.0295 | −1.5594 |
| VI | 2-Carboxybenzalpyruvic acid | C11H7O5- | 219 | 5.9 | – | 219.0303 | 219.0299 | 1.7901 |
| VII | 2-Carboxybenzaldehyde | C8H5O3- | 149 | 5.8 | 5.7 | 149.0242 | 149.0244 | −1.3027 |
| VIII | Phthalic acid | C8H5O4- | 165 | 4.4 | 4.1 | 165.0191 | 165.0193 | −1.1700 |
a
–, not analyzed.