Figure 1.
Comparison of the binding of DNA oligomer and polyamides designed to recognize TTCCA repeats in metaphase chromosomes from a normal male. (A) Conventional FISH of the 20-nt (TTCCA)4 oligomer to denatured chromosome preparations. Hybridization sites are labeled with avidin–fluorescein (pseudocolored red), and chromosomes are counterstained with DAPI (gray). (B and D) Binding of the polyamides S-PA-F and L-PA-F to non-denatured chromosomes, respectively. The polyamides are responsible for all visible signals (intensity displayed as gray scale). (C and E) Relative intensity profile plots for chromosomes 9, Y and 1 from (B) and (D), respectively. The plotted intensities represent the sum of signal intensities in a seven-pixel-wide swath running from the p-terminus to q-terminus for each chromosome.