Figure 5:

Prenylomic results for novel GGPP analogues and comparisons of C15hAlkOPP to C15AlkOPP. (A-D) Volcano plots generated from a t-test analysis of the normalized TMT reporter ion intestines with an FDR=1%, S⁰ =0.5. Volcano plots showing the fold change of proteins labeled with (A) C15hAlkOPP compared to GGPP, (B) C20AlkOPP compared to FPP, (C) C15hAlkOH compared to FOH. (D) C20AlkOH compared to FOH. (E-F) Venn diagrams showing the overlap of ungrouped enriched proteins found when comparing (E) C15hAlkOPP to C15hAlkOH or (F) C20AlkOPP to C20AlkOH. (G) Correlation of the Log₂ fold change of the proteins found both with C15hAlkOPP and C20AlkOPP. Rank spearman correlation was performed on the 6 proteins shared between the two datasets. The spearman correlation coefficient is −0.1, with a p-value 0.4. Solid black line illustrates perfect positive correlation (H) Venn diagram of all (left) ungrouped proteins found enriched in C15AlkOPP or C15hAlkOPP and a Venn diagram of only the farnesylated proteins found with C15AlkOPP or C15hAlkOPP. *ALDH9A1. (I) Correlation of 37 proteins found with both C15AlkOPP and C15hAlkOPP. The spearman correlation coefficient is −0.1, with a p-value = 0.4. The solid black line shows the ideal positive correlation.