Figure 8:

Western blot analysis of H-Ras protein in cells subjected to statin-induced inhibition of prenylation and rescue with different isoprenoid diphosphates and alcohol precursors. COS-7 cells were treated with (A) 10 μM or (B) 25 μM lovastatin for 1.5 h (except for the sample indicated by -) followed by supplementation with different isoprenoids for an additional 18 h while retaining the statin. Cells were then lysed, the lysates fractionated via SD-PAGE, blotted to nitrocellulose membranes and visualized via western blotting followed by imaging.