. Author manuscript; available in PMC: 2026 Jan 7.

Published in final edited form as: Biochemistry. 2024 Dec 9;64(1):138–155. doi: 10.1021/acs.biochem.4c00558

Table 1:

Kinetic parameters for probes evaluated using rFTase and rGGTase

	rFTase and Ds-GCVLS					rGGTase and Ds-GCVLL
Analogue	k_cat (s⁻¹)	K_M (μM)	k_cat /K_M (M⁻¹s⁻¹)	k_cat rel. to FPP (%)	k_cat /K_M rel. to FPP (%)	k_cat (s⁻¹)	K_M (μM)	k_cat /K_M (M⁻¹s⁻¹)	k_cat rel. to GGPP (%)	k_cat/K_M rel. to GGPP (%)
FPP^a	0.3	1.5	2.0 × 10⁵	100	100	-	-	-	-	-
C10AlkOPP^b	-	-	2.4 × 10⁴	-	12	-	-	-	-	-
C15AlkOPP^c	0.037 ± 0.002	0.69 ± 0.15	5.4 × 10⁴	12	27	0.02	3.0	6.7 × 10³	38	22
C15hAlkOPP	0.028 ± 0.001	0.26 ± 0.06	1.1 × 10⁵	9	55	0.032 ± 0.0006	0.084 ± 0.02	3.8 × 10⁵	62	122
GGPP^d	ns^e	ns	ns	ns	ns	0.052	0.17	3.1 × 10⁵	100	100
C20AlkOPP	~0.0018			<1	-	0.030 ± 0.0009	0.35 ± 0.06	8.5 × 10⁴	58	27

Data previously reported in [⁴⁶] for FPP with rFTase.

Data previously reported in [⁴⁴], found with rFTase and Ds-TKCVLS.

Data previously reported with the C15AlkOPP probe with hGGTase I and Ds-GCVLL in [⁴⁰].

Data previously reported in [⁴⁷].

GGPP has previously been reported as an inhibitor of FTase and not a substrate.^48,49 A dash (−) indicates that no kinetic measurements were performed.