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. 2002 Jul 1;30(13):2727–2735. doi: 10.1093/nar/gkf413

Figure 3.

Figure 3

ATPase– Rad54 enhances rates of spontaneous direct repeat deletion and allelic gene conversion. Spontaneous HR yielding Ura+ recombinants was assayed by fluctuation analysis of 11 independent parental colonies of JW3084 (Direct Repeats) or JC3520 (Allelic). For direct repeats, Ura+ products were replica plated to leucine omission medium to determine rates for Ura+ Leu+ (gene conversion) and Ura+ Leu– (deletion). Cells carried an empty (TRP1) vector as control, or they overexpressed wild-type (RAD54) or ATPase– Rad54 (rad54-KR and rad54-KA).