Fig. 3.

Effects of cytokine gene adjuvants on the quantity of IFN-γ-specific CD8⁺ T cells and mRNA levels of granzyme B in isolated splenocytes. (A) The quantity of IFN-γ-secreting E7-specific CD8⁺ T cells was assessed using ELISPOT. The spot counts were presented as the mean ± SE for 4 mouse samples per vaccinated group, normalized to 5 × 10⁵ splenocytes. (B) Representative ELISPOT images from each vaccinated group were shown. (C) The mRNA levels of specific granzyme B were analyzed via real-time PCR and normalized relative to GAPDH. *:P<0.05, ***:P<0.001, ****:P<0.0001