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Demonstration of the contrast improvement in synchrotron X-PCI and sample shrinkage due to ethanol dehydration between (a) native heart kept in formalin, (b) 9 h after and (b) 342 h after immersion in 70% ethanol solution. (d) Distribution of pixel intensities in the three datasets. All the datasets were reconstructed using the same contrast limits. Dashed lines in panel (a) indicate the three apico-basal slices selected for quantification.
