Figure 10.

The activity of GGT-I in era1-2 mutants. A GFP-BDCaM53 fusion protein was transiently expressed in leaves of wt Col-0 and era1-2 mutants following transformation by biolistic bombardments. The intracellular localization of the fusion protein was determined by imaging cell with confocal microscope. Prenylated GFP-BDCaM53 protein was located to the plasma membrane resulting in green fluorescence at the periphery of the cells. Un-prenylated fusion protein accumulated in the nucleus (N; Rodríguez-Concepción et al., 1999b). The red fluorescence came from plastids (P). Bars are 20 microns.