Figure 7.

Substrate saturation curves for prenylation of GST-BDCaM53 and GST-CTIL by GGT-I. Prenylation reactions were carried out with concentrations of GST-BDCaM53 and GST-CTIL as indicated in the figure. All other conditions were as described in “Materials and Methods.” Reactions were carried out in triplicates, and were terminated by separating between protein-incorporated and free GGPP using the acidic ethanol method (“Materials and Methods”). Each graph point represents the average value of each of the three reactions and bars are sd values. To express the incorporation of GGPP in values of pmol min⁻¹, the radioactivity of known amount of [³H]GGPP was measured by scintillation counting.