Figure 8.

How does GGT-I activity depend on temperature? Prenylation reactions of CaM53 were carried out at different temperatures, as indicated on the figure. All other conditions were as described (“Materials and Methods”). Separation of the protein products on SDS gels terminated reactions, followed by fluorography of gels and exposure to x-ray films (A). Values for each band pair were averaged, and the maximal intensity was given a value of 1. −, Denotes reactions carried out with boiled GGT-I enzyme; +, reactions carried out with active GGT-I.