Figure 9.

Regulation of GGT-I by pH. Prenylation reactions of GST-BDCaM53 were carried out at different pH values as indicated on the figure. All other conditions were as described (“Materials and Methods”). Reactions were carried out in triplicates, and were terminated by separating between protein-incorporated and free GGPP using the acidic ethanol method (“Materials and Methods”). Each graph point represents the average value of each of the three reactions and bars are sd values. The incorporation of GGPP into substrate protein was calculated as described in Figure 7.