Fig. 3.

Subcutaneous implantation to evaluate cell infiltration in decellularized tissue-engineered vascular grafts with 0-, 15-, 30- and 45-min elastase treatment. A) H&E, EVG and DAPI staining of 0-, 15-, 30- and 45-min elastase-treated dTEVGs, the dotted line showing the structure of the grafts (scale bar, 50 μm). B) Number of cells infiltrating into the graft per high-power field in 0-, 15-, 30- and 45-min elastase-treated dTEVGs (n = 3, non-significant difference is not marked). C) Schematic illustrating the process of implantation control group and elastase-treated groups into the subcutaneous tissue of the rat. DTEVG: decellularized tissue-engineered vascular graft. Data are represented as mean ± standard deviations. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.