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. 2024 Dec 12;30:101402. doi: 10.1016/j.mtbio.2024.101402

Fig. 5.

Fig. 5

Evaluation of cell infiltration and remodeling in mildly elastase-treated decellularized tissue-engineered vascular grafts in rat arteriovenous graft models. A) H&E and EVG staining of dTEVGs with or without mild elastase treatment after transplantation into rat AVG models for 42 days. B) Immunofluorescence staining of CD31 at day 42 between dTEVGs with or without mild elastase treatment. C) The proportion of reendothelialization in the whole lumen of dTEVGs with or without mild elastase treatment (n = 4). D) Immunofluorescence staining of α-SMA and CNN1 at day 42 between dTEVGs with or without mild elastase treatment. E) The number of α-SMA- and CNN1-positive cells in dTEVGs with or without mild elastase treatment (n = 4). F) Immunofluorescence staining of CD68 at day 42 between dTEVGs with or without mild elastase treatment. G) The number of CD68-positive cells in dTEVGs with or without mild elastase treatment (n = 3). The dotted line showing the structure of the grafts. Scale bar, 50 μm. Data are represented as mean ± standard deviations. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.