Figure 10.

The effect of prewashing C. communis epidermal strips on the subsequent response of the stomata that they contain to temperature in 0.2 cm³ 65 mm KCl. Stomata were allowed to open in the light (350 μmol m⁻² s⁻¹) in one group of 20 and one group of five epidermal strips floating on two petri dishes of 25 cm³ 65 mm KCl at RT for 1 h. After 1 h the smaller group to be “washed” were rinsed (floated for 30 s on 25cm³ fresh KCl) and transferred to a second dish containing 25 cm³ fresh 65 mm KCl at RT, and the larger group was left where it was. All were incubated for a further hour in the light. Finally, the strips were transferred to the appropriate treatment solutions described above for 1 h before measuring stomatal apertures (means of 25 stomata per strip in five strips).