Purification of soybean phytase. a, Purification
table. b, SDS-polyacrylamide electrophoresis of protein samples from
protein purification steps. 1, Crude extract; 2, heat shock
precipitation; 3, ammonium sulfate precipitation; 4, cation exchange
chromatography; 5, lectin affinity chromatography; 6, metal chelate
chromatography; 7, phy 1.1 from monoQ chromatography. Approximately 1
μg of protein was loaded in each lane.