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Purification of soybean phytase. a, Purification table. b, SDS-polyacrylamide electrophoresis of protein samples from protein purification steps. 1, Crude extract; 2, heat shock precipitation; 3, ammonium sulfate precipitation; 4, cation exchange chromatography; 5, lectin affinity chromatography; 6, metal chelate chromatography; 7, phy 1.1 from monoQ chromatography. Approximately 1 μg of protein was loaded in each lane.
