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Effects of temperature and pH on soybean phytase activity. a, Activity of purified soybean phytase was assayed at temperatures ranging from 34°C to 78°C. b, Thermostability of the purified phytase was determined by preincubating samples at temperatures ranging from 40°C to 100°C for 10 min and subsequently assaying remaining phytase activity. c, Activity of the purified soybean phytase was assayed from pH 2.5 to 7.0.
