Table I.
Comparison of GmPhy with homologous plant sequences
| Source | Accession No. | Identity | Consensus
Motifsa
|
||||
|---|---|---|---|---|---|---|---|
| I DXG | II GDXXY | III GNH(E,D) | IV VXXH | V GHXH | |||
| % | |||||||
| GmPhy | AF272346 | 100.0 | DLG | GDVTY | GNHE | VTWH | GHVH |
| At1 | AC012395 | 74.1 | DLG | GDVSY | GNHE | ASWH | GHVH |
| At2 | AC003974 | 51.6 | DLG | GGFSY | GEHE | ATWS | SHVD |
| At3 | AL035528 | 51.3 | DLG | GDLTY | GNHE | ATMH | GHVH |
| At4 | AL353912 | 32.3 | DLG | GDLSY | GNHE | AVVH | GHVH |
| At5 | AL353912 | 32.9 | DLG | GDLSY | GNHE | VLLH | GHVH |
| At6 | AL353912 | 32.5 | DLG | GDLSY | GNHE | VVMH | GHVH |
| At7 | AL022141 | 26.8 | DLG | GDLSY | GNHE | VMVH | GHVH |
| At8 | AC007212 | 23.4 | DLG | GDLSY | GNYE | VLVH | GHVH |
| At9 | AC019018 | 24.5 | DLG | GDLSY | GNHE | VLVH | GHVH |
| At10 | AC007290 | 27.9 | DLG | GDLSY | GNHE | VLVH | GHVH |
| At11 | AC007047 | 27.1 | DLG | GDISY | GNHE | VLMH | GHVH |
| IbPAP1 | AF200825 | 25.9 | DIG | GDLSY | GNHE | VLVH | GHVH |
| IbPAP2 | AF200826 | 28.5 | DLG | GDLSY | GNHE | VLMH | GHVH |
| IbPAP3 | AJ006870 | 30.5 | DLG | GDLSY | GNHE | VLMH | GHVH |
| PvPAP1 | AJ001270 | 27.5 | DLG | GDLSY | GNHE | VLMH | GHVH |
| GmPAP1 | AF200824 | 27.1 | DLG | GDLSY | GNHE | VLMH | GHVH |
| Sp1 | AB039746 | 26.8 | DLG | GDLSY | GNHE | VLMH | GHVH |
| La1 | AB023385 | 20.6 | DLG | GDLSY | GNHE | VLVH | GHVH |
| Ao1 | AF126255 | 25.7 | DLG | GDLSY | GNHE | VLMH | GHVH |
The source and accession no. of the top scoring sequences from the BLAST search, and percentage identity of the predicted amino acid sequences to the soybean phytase sequence (as determined by the Megalign sequence alignment program in DNAStar) are shown. The amino acids sequences from regions corresponding to the conserved motifs are listed for each plant sequence.
a
Motifs as described by Schenk et al. (2000).