Table II.
Expression of reporter proteins with N-terminal modifications
| Name of Construct | Activity | Fold Activation |
|---|---|---|
| GUS constructs | ||
| (i) M-GUS | 14.6a (±1.2) | 1.0 |
| (ii) MA-GUS | 59.9 (±4.1) | 4.1 |
| (iii) MAS1-GUS | 181.6 (3.9) | 12.4 |
| (iv) MAS1S-GUS | 603.0 (±13.8) | 41.2 |
| (v) MDS1S-GUS | 4.2 (±0.3) | 0.3 |
| (vi) MACS-GUS | 44.4 (±2.6) | 3.1 |
| (vii) MAS2S-GUS | 110.2 (±2.8) | 7.6 |
| (viii) MAS3S-GUS | 100.6 (±1.5) | 6.9 |
| (ix) MAS4S-GUS | 86.3 (±3.6) | 5.9 |
| GFP constructs | ||
| (i) M-GFP | 16.3b (±1.1) | 1.0 |
| (ii) MAS1S-GFP | 345.5 (±9.7) | 21.2 |
a
GUS ± sd (glucuronidase activity expressed as ×103 pmol/h/mg protein).
b
GFP ± sd (GFP activity expressed as ×102 relative fluorescence/mg protein).