Appendix 3.

Frequency of other mutant alleles of the pfdhfr-ts gene in blood

			Blood (N=86)
Codons	Strains and mutations observed		Numbers	Proportions (%)
			Blood (n=65)
dhfr_51	Wild type (N)	Asn-51	10	15,38
	Mutants	Ile-51	40	61,50
		Phe-51	7	10,76
		Lys-51	1	1,53
		Leu-51	3	4,61
		Pro-51	3	4,61
		Ser-51	1	1,53
		Met-51	0	0
		Thr-51	0	0
		Val-51	0 (0)	0
			Blood (n=66)
dhfr_59	Wild type (C)	Cys-59	10	15,15
	Mutants	Arg-59	36	54,54
		Ala-59	1	1,51
		Gly-59	11	16,66
		Leu-59	1	1,51
		Ser-59	5	7,57
		Trp-59	2	3,03
		Pro-59	0	0
		Asn-59	0	0
		Val-59	0	0
		Phe-59	0	0
		Tyr-59	0	0
			Blood (n=81)
dhfr_108	Wild type (S)	Ser-108	14	17,28
	Mutants	Asn-108	60	74,07
		Ala-108	2	2,46
		Phe-108	1	1,23
		His-108	2	2,46
		Thr-108	1	1,23
		Val-108	1	1,23
		Asp-108	0	0
		Lys-108	0	0
		Gly-108	0	0
		Pro-108	0	0
		Ile-108	0	0
		Arg-108	0	0