Table 3.
Summary of sequencing of DNA extracted from isolates according to mutations.
| Sequenced frag- ments (N*= 94) | Changes | Success n**(%) | Ches n** (%) |
|---|---|---|---|
| Blood (n=86) | |||
| Asn-51-Ile | 65 (75,60%) | 21 (24,4%) | |
| Pfdhfr (N*=86) | Cys-59-Arg | 66 (76,75%) | 20 (23,25%) |
| Ser-108-Asn | 81 (94,20%) | 5 (5,80%) | |
| Blood (n=74) | |||
| Met-74-Ile | 59 (79,73%) | 15 (20,27%) | |
| Pfcrt (N*=74) | Asn-75-Glu | 64 (84,50%) | 10 (13,50%) |
| Lys-76-Thr | 64 (84,50%) | 10 (13,50%) | |
| Blood (n=93) | |||
| Tyr-493-Ile | 79 (84,94%) | 14 (15,06%) | |
| pfk13 propeller (N*=93) | Arg-539-Thr | 85 (91,40%) | 8 (8,60%) |
| Ile-543-Thr | 89 (95,70%) | 4 (4,30%) | |
| Cys-580-Tyr | 93 (100%) | 0 (0,0%) | |
| Met-476-Ile | 77 (82,80%) | 16 (17,20%) | |
| Arg-561-His | 88 (94,60%) | 5 (5,40%) |
*N represents the total number of isolates successfully sequenced at the three sites.
**n represents the number of isolates for which codons of interest of the sequence were located.