Phylogenetic tree of the Arabidopsis CNGC
transporters. Entries in the Munich Information Center for Protein
Sequences Arabidopsis database (MATDB) were compared with available
corresponding cDNA entries in the National Center for Biotechnology
Information database, to minimize errors for each predicted
protein sequence. By default, the MATDB predicted protein sequences
were used for final alignment. Exceptions are: accession no. AAF97331.1
for At1g01340, accession no. CAB40128.1 for At2g46430, accession no.
AAF73129.1 for At3g17690, and accession no. AAF73128.1 for At3g17700.
Bold lines indicate that protein sequences predicted from cDNAs are
available. Final protein alignment, tree drawing, and bootstrap
analysis were done with ClustalX (Higgins and Sharp, 1988), and the
tree was drawn using Treeview. CNGCs 1 through 6 have already been
named in the literature (Köhler et al., 1999). To generate a
uniform nomenclature, ACBK1, CNBT1, and CNBT2 are assigned the names
CNGC10, 20, and 19, respectively, and the remaining genes are also
assigned systematic names. Values indicate the number of times (in
percent) that each branch topology was found during bootstrap
analysis.