Table I.

Pumpkin growth, distribution of B, and dRG-II-B ratio in pumpkin plants

Treatment	Leaf No.	Fresh Leaf Wt	Leaf B	AIR Ba	dRG-II-B in Total RG-IIb
		mg	μg g−1 dry leaf		%
25 μm B	Cotyledon	660 ± 38	99.3 ± 5.2	32.9 ± 2.4	85
	First leaf	858 ± 62	69.5 ± 1.3	19.7 ± 1.7	82
	Second leaf	546 ± 43	39.3 ± 2.7	11.0 ± 0.6	87
	Third leaf	241 ± 44	32.5 ± 2.2	15.3 ± 1.9	86
	Fourth leaf	26 ± 4	32.0 ± 5.0	14.7 ± 4.2	82
0 μm B	Cotyledon	751 ± 40	92.4 ± 6.1	41.3 ± 1.8	93
	First leaf	530 ± 50	21.5 ± 1.2	19.1 ± 4.9	88
	Second leaf	216 ± 30	4.2 ± 1.6	3.8 ± 0.1	35
	Third leaf	131 ± 20	3.0 ± 1.0	3.0 ± 0.1	10
	Fourth leaf	37 ± 14	2.6 ± 0.3	2.7 ± 0.1	20

Plants were precultured for 7 d in 10 μm B. Seven days prior to harvest, one-half of the plants were transferred to B-free solution and the remaining one-half were transferred to 25 μm B. Fresh leaf wt was expressed as means ± se (n = 8). Alcohol-insoluble residue (AIR) were isolated from each leaf (details in “Materials and Methods”). B was determined in the leaf and AIR samples by inductively coupled plasma-mass spectrometry (ICP-MS). Data are means of three replicates and se are shown.

^a AIR B was calculated from the B content in AIR determined by ICP-MS and the yield of AIR. 

^b The relative proportion of dRG-II-B to total RG-II was determined by size exclusion chromatography (SEC)/refractive index detector of the endo-polygalacturonase (EPG) digests of the AIR. Data are average from two individual experiments.