Table III.
Glycosyl residue compositions of the mRG-II and dRG-II-B isolated from pumpkin leaf in the presence or absence of boric acid and 10B-treated pumpkin
| Glycosyl Residue | B Normala
|
B-Deficientb mRG-IId |
10B Treatedc
|
||
|---|---|---|---|---|---|
| dRG-II-Bd | mRG-IId | dRG-II-Bd | mRG-IId | ||
| 2-Me Fuc | 3 | 3 | 1 | 2 | 2 |
| Rha | 7 | 6 | 12 | 7 | 5 |
| Fuc | 2 | 2 | 2 | 2 | 1 |
| 2-Me Xyl | 4 | 3 | 2 | 2 | 2 |
| Ara | 9 | 12 | 12 | 12 | 14 |
| Api | 5 | 6 | 7 | 8 | 8 |
| AcerA | 3 | 3 | 4 | 4 | 4 |
| Gal | 10 | 9 | 11 | 12 | 13 |
| GalA | 43 | 38 | 39 | 35 | 40 |
| GlcA | 5 | 5 | 3 | 4 | 2 |
| Kdo | 4 | 8 | 4 | 5 | 4 |
| Dha | 5 | 7 | 3 | 7 | 5 |
| 10B/(10B +11B)e | 0.19 | –f | –f | 0.73 | –f |
AIR was isolated from the second to the fourth leaves. RG-II was isolated by SEC from EPG digests of the AIR of pumpkin leaf grown in the presence (25 μm) or absence of boric acid and 10B-treated leaf. The neutral and acidic glycosyl residues were determined by gas-liquid chromatography analysis of alditol acetates and trimethyl silyl derivatives, respectively.
Pumpkin grown in the presence of B boric acid (25 μm).
Pumpkin grown in the absence of boric acid.
B-deficient pumpkins that were grown for 16 h in the presence of 10B-enriched boric acid (25 μm).
dRG-II-B and mRG-II were isolated from the EPG digest of the AIR by SEC.
The relative abundance of 11B and 10B in dRG-II-B were determined by SEC/ICP-MS analysis of the EPG digest of the AIR.
mRG-II contains no detectable amounts of B.