Table IV.
Glycosyl linkage composition of the mRG-II and dRG-II-B isolated from pumpkin grown in the presence or absence of boric acid and 10B-treated pumpkin
| Glycosyl Linkage | B Normala
|
B-Deficientb mRG-IId |
10B Treatedc
|
||
|---|---|---|---|---|---|
| dRG-II-Bd | mRG-IId | dRG-II-Bd | mRG-IId | ||
| T-Rha | 3 | 4 | 2 | 5 | 4 |
| 2-Rha | 1 | 2 | 7 | 1 | 2 |
| 3-Rha | 5 | 5 | 3 | 5 | 5 |
| 2, 4-Rha | – | – | 1 | – | – |
| 2, 3, 4-Rha | 7 | 6 | 8 | 4 | 4 |
| T-Fuc | 3 | 4 | 3 | 4 | 3 |
| 3, 4-Fuc | 5 | 5 | 4 | 5 | 4 |
| T-Araf | 7 | 7 | 6 | 7 | 10 |
| T-Arap | 3 | 4 | 2 | 5 | 5 |
| 2-Arap | 1 | 1 | 1 | 1 | 1 |
| T-Xyl | 5 | 6 | 5 | 6 | 5 |
| 3′-Api | 7 | 10 | 10 | 8 | 12 |
| 2, 3, 3′-Api | 5 | – | – | 6 | – |
| T-Gal | 5 | 5 | 5 | 6 | 6 |
| 2, 4-Gal | 4 | 4 | 3 | 4 | 5 |
| 2-GlcA | 5 | 7 | 4 | 6 | 5 |
| T-GalA | 13 | 13 | 12 | 7 | 8 |
| 4-GalA | 5 | 6 | 9 | 8 | 8 |
| 3, 4-GalA | 4 | 3 | 4 | 4 | 4 |
| 2, 4-GalA | 5 | 3 | 4 | 4 | 4 |
| 2, 3, 4-GalA | 8 | 5 | 7 | 4 | 5 |
AIR was isolated from the second to the fourth leaves. RG-II was isolated from EPG digest of the AIR of pumpkin leaf grown in the presence (25 μm) or absence of boric acid and 10B-treated leaf.
a
Pumpkin grown in the presence of B boric acid (25 μm).
b
Pumpkin grown in the absence of boric acid.
c
B-deficient pumpkins that were grown for 16 h in the presence of 10B-enriched boric acid (25 μm).
d
dRG-II-B and mRG-II were isolated from the EPG digest of the AIR by SEC.