Figure 4.

Localization of NtmybAS mRNA during tobacco anther development. Paraffin-embedded sections were hybridized with digoxygenin-labeled NtmybAS1 probes and viewed under bright field. Scale bar = 400 μm. Magnification ×100 in 8- to 9-mm and 10- to 12-mm buds; and ×40 in 13- to 15-mm, 16- to 20-mm, and 21- to 30-mm buds. a through e, NtmybAS1 antisense probe with anthers from: a, 8 to 9 mm; b, 10 to 12 mm; c, 13 to 15 mm; d, 16 to 20 mm; and e, 21- to 30-mm buds. Staining represents hybridization signal. f through j, NtmybAS1 sense probe with anthers from: f, 8 to 9 mm; g, 10 to 12 mm; h, 13 to 15 mm; i, 16 to 20 mm; and j, 21- to 30-mm buds.